2021
van Daal, R. J. J.; Aydin, Ç.; Michon, F.; Aarts, A. A. A.; Kraft, M.; Kloosterman, F.; Haesler, S.
Implantation of Neuropixels probes for chronic recording of neuronal activity in freely behaving mice and rats Journal Article
In: Nature Protocols, vol. 16, no. 7, pp. 3322–3347, 2021.
Abstract | Links | BibTeX | Altmetric | Dimensions | PlumX | Tags: brain implant, neural probe, neuropixels, neurotechnology
@article{pmid34108732,
title = {Implantation of Neuropixels probes for chronic recording of neuronal activity in freely behaving mice and rats},
author = {R. J. J. van Daal and Ç. Aydin and F. Michon and A. A. A. Aarts and M. Kraft and F. Kloosterman and S. Haesler},
doi = {10.1038/s41596-021-00539-9},
year = {2021},
date = {2021-07-01},
urldate = {2021-07-01},
journal = {Nature Protocols},
volume = {16},
number = {7},
pages = {3322--3347},
abstract = {How dynamic activity in neural circuits gives rise to behavior is a major area of interest in neuroscience. A key experimental approach for addressing this question involves measuring extracellular neuronal activity in awake, behaving animals. Recently developed Neuropixels probes have provided a step change in recording neural activity in large tissue volumes with high spatiotemporal resolution. This protocol describes the chronic implantation of Neuropixels probes in mice and rats using compact and reusable 3D-printed fixtures. The fixtures facilitate stable chronic in vivo recordings in freely behaving rats and mice. They consist of two parts: a covered main body and a skull connector. Single-, dual- and movable-probe fixture variants are available. After completing an experiment, probes are safely recovered for reimplantation by a dedicated retrieval mechanism. Fixture assembly and surgical implantation typically take 4-5 h, and probe retrieval takes ~30 min, followed by 12 h of incubation in probe cleaning agent. The duration of data acquisition depends on the type of behavioral experiment. Since our protocol enables stable, chronic recordings over weeks, it enables longitudinal large-scale single-unit data to be routinely obtained in a cost-efficient manner, which will facilitate many studies in systems neuroscience.},
keywords = {brain implant, neural probe, neuropixels, neurotechnology},
pubstate = {published},
tppubtype = {article}
}
Steinmetz, N. A.; Aydin, C.; Lebedeva, A.; Okun, M.; Pachitariu, M.; Bauza, M.; Beau, M.; Bhagat, J.; hm, C.; Broux, M.; Chen, S.; Colonell, J.; Gardner, R. J.; Karsh, B.; Kloosterman, F.; Kostadinov, D.; Mora-Lopez, C.; O’Callaghan, J.; Park, J.; Putzeys, J.; Sauerbrei, B.; Daal, R. J. J.; Vollan, A. Z.; Wang, S.; Welkenhuysen, M.; Ye, Z.; Dudman, J. T.; Dutta, B.; Hantman, A. W.; Harris, K. D.; Lee, A. K.; Moser, E. I.; O’Keefe, J.; Renart, A.; Svoboda, K.; usser, M.; Haesler, S.; Carandini, M.; Harris, T. D.
Neuropixels 2.0: A miniaturized high-density probe for stable, long-term brain recordings Journal Article
In: Science, vol. 372, no. 6539, 2021.
Links | BibTeX | Altmetric | Dimensions | PlumX | Tags: brain implant, neuropixels, neurotechnology
@article{pmid33859006,
title = {Neuropixels 2.0: A miniaturized high-density probe for stable, long-term brain recordings},
author = {N. A. Steinmetz and C. Aydin and A. Lebedeva and M. Okun and M. Pachitariu and M. Bauza and M. Beau and J. Bhagat and C. hm and M. Broux and S. Chen and J. Colonell and R. J. Gardner and B. Karsh and F. Kloosterman and D. Kostadinov and C. Mora-Lopez and J. O'Callaghan and J. Park and J. Putzeys and B. Sauerbrei and R. J. J. Daal and A. Z. Vollan and S. Wang and M. Welkenhuysen and Z. Ye and J. T. Dudman and B. Dutta and A. W. Hantman and K. D. Harris and A. K. Lee and E. I. Moser and J. O'Keefe and A. Renart and K. Svoboda and M. usser and S. Haesler and M. Carandini and T. D. Harris},
doi = {10.1126/science.abf4588},
year = {2021},
date = {2021-00-01},
urldate = {2021-00-01},
journal = {Science},
volume = {372},
number = {6539},
keywords = {brain implant, neuropixels, neurotechnology},
pubstate = {published},
tppubtype = {article}
}