@article{pmid34108732,

title = {Implantation of Neuropixels probes for chronic recording of neuronal activity in freely behaving mice and rats},

author = {R. J. J. van Daal and Ç. Aydin and F. Michon and A. A. A. Aarts and M. Kraft and F. Kloosterman and S. Haesler},

doi = {10.1038/s41596-021-00539-9},

year  = {2021},

date = {2021-07-01},

urldate = {2021-07-01},

journal = {Nature Protocols},

volume = {16},

number = {7},

pages = {3322--3347},

abstract = {How dynamic activity in neural circuits gives rise to behavior is a major area of interest in neuroscience. A key experimental approach for addressing this question involves measuring extracellular neuronal activity in awake, behaving animals. Recently developed Neuropixels probes have provided a step change in recording neural activity in large tissue volumes with high spatiotemporal resolution. This protocol describes the chronic implantation of Neuropixels probes in mice and rats using compact and reusable 3D-printed fixtures. The fixtures facilitate stable chronic in vivo recordings in freely behaving rats and mice. They consist of two parts: a covered main body and a skull connector. Single-, dual- and movable-probe fixture variants are available. After completing an experiment, probes are safely recovered for reimplantation by a dedicated retrieval mechanism. Fixture assembly and surgical implantation typically take 4-5 h, and probe retrieval takes ~30 min, followed by 12 h of incubation in probe cleaning agent. The duration of data acquisition depends on the type of behavioral experiment. Since our protocol enables stable, chronic recordings over weeks, it enables longitudinal large-scale single-unit data to be routinely obtained in a cost-efficient manner, which will facilitate many studies in systems neuroscience.},

keywords = {brain implant, neural probe, neuropixels, neurotechnology},

pubstate = {published},

tppubtype = {article}

}

@article{pmid31791021,

title = {System for recording from multiple flexible polyimide neural probes in freely behaving animals},

author = {R. J. J. van Daal and J. J. Sun and F. Ceyssens and F. Michon and M. Kraft and R. Puers and F. Kloosterman},

doi = {10.1088/1741-2552/ab5e19},

year  = {2020},

date = {2020-02-01},

urldate = {2020-02-01},

journal = {Journal of Neural Engineering},

volume = {17},

number = {1},

pages = {016046},

keywords = {brain implant, neural probe, neurotechnology},

pubstate = {published},

tppubtype = {article}

}

@article{pmid29048396,

title = {Time Multiplexed Active Neural Probe with 1356 Parallel Recording Sites},

author = {B. C. Raducanu and R. F. Yazicioglu and C. M. Lopez and M. Ballini and J. Putzeys and S. Wang and A. Andrei and V. Rochus and M. Welkenhuysen and N. V. Helleputte and S. Musa and R. Puers and F. Kloosterman and C. V. Hoof and R. Fiath and I. Ulbert and S. Mitra},

doi = {10.3390/s17102388},

year  = {2017},

date = {2017-00-01},

urldate = {2017-00-01},

journal = {Sensors},

volume = {17},

number = {10},

keywords = {neural probe, neurotechnology},

pubstate = {published},

tppubtype = {article}

}

@article{pmid27351591,

title = {Integration of silicon-based neural probes and micro-drive arrays for chronic recording of large populations of neurons in behaving animals},

author = {F. Michon and A. Aarts and T. Holzhammer and P. Ruther and G. Borghs and B. McNaughton and F. Kloosterman},

doi = {10.1088/1741-2560/13/4/046018},

year  = {2016},

date = {2016-00-01},

urldate = {2016-00-01},

journal = {Journal of Neural Engineering},

volume = {13},

number = {4},

pages = {046018},

keywords = {brain implant, neural probe, neurotechnology},

pubstate = {published},

tppubtype = {article}

}

@article{pmid12034331,

title = {Recording and marking with silicon multichannel electrodes},

author = {G. Townsend and P. Peloquin and F. Kloosterman and J. F. Hetke and L. S. Leung},

doi = {10.1016/s1385-299x(02)00139-3},

year  = {2002},

date = {2002-00-01},

urldate = {2002-00-01},

journal = {Brain Research Protocols},

volume = {9},

number = {2},

pages = {122--129},

keywords = {neural probe},

pubstate = {published},

tppubtype = {article}

}